Hotspot

trnS-GCU-trnG-GCC

LSC · 8088-8876 · 789 bp

Back to Saxifraga

Pi 0.0749

MarkerSeek score 75.10

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 789
variable_sites: 334
pi: 0.0749
unique_haplotype_count: 100
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	491-932 bp	1.0	63.7
trnS-GCU-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	490-931 bp	1.0	63.7
trnS-GCU-trnG-GCC_p3	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.0	57.3	494-935 bp	1.0	62.6
trnS-GCU-trnG-GCC_p4	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	493-934 bp	1.0	62.6
trnS-GCU-trnG-GCC_p5	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	492-933 bp	1.0	62.6