Hotspot

psbK-psbI

LSC · 7362-7754 · 393 bp

Back to Saxifraga

Pi 0.0596

MarkerSeek score 74.00

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 393
variable_sites: 172
pi: 0.0596
unique_haplotype_count: 98
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CCAAATTACCCGAGGCCTAC	ACGAAGAGTTTGAGAGTAAGCA	55.0	40.9	58.0	57.4	422-542 bp	1.0	71.0
psbK-psbI_p2	TATGCCGGTCATACCTTTGC	ACGAAGAGTTTGAGAGTAAGCA	50.0	40.9	58.0	57.4	376-496 bp	1.0	71.0
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	ACGAAGAGTTTGAGAGTAAGCA	50.0	40.9	57.9	57.4	336-456 bp	1.0	70.9
psbK-psbI_p4	CCGGTCATACCTTTGCTGTT	ACGAAGAGTTTGAGAGTAAGCA	50.0	40.9	58.2	57.4	372-492 bp	0.982759	69.2
psbK-psbI_p5	TACCCGAGGCCTACTCTTTT	ACGAAGAGTTTGAGAGTAAGCA	50.0	40.9	57.8	57.4	416-536 bp	0.974138	68.3