Hotspot

rps16-trnQ-UUG

LSC · 6066-6722 · 657 bp

Back to Saxifraga

Pi 0.0702

MarkerSeek score 76.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 657
variable_sites: 293
pi: 0.0702
unique_haplotype_count: 95
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TGGTGTTTCCTTGTTCCAGG	GCAAAGGTATGACCGGCATA	50.0	50.0	57.9	58.0	1558-2141 bp	0.948276	89.1
rps16-trnQ-UUG_p2	TGGTGTTTCCTTGTTCCAGG	GTAGGCCTCGGGTAATTTGG	50.0	55.0	57.9	58.0	1512-2095 bp	0.931034	88.4
rps16-trnQ-UUG_p3	TGGTGTTTCCTTGTTCCAGG	CAACCCACTCACCCAATCAA	50.0	50.0	57.9	58.0	1317-1896 bp	0.844828	85.2
rps16-trnQ-UUG_p4	TGGGTTCTAGTCGAGCAGAA	CAACCCACTCACCCAATCAA	50.0	50.0	58.1	58.0	915-1486 bp	0.818966	84.1
rps16-trnQ-UUG_p5	CGCGTGTTCCACTTTTTGTT	CAACCCACTCACCCAATCAA	45.0	50.0	58.1	58.0	1361-1390 bp	0.060345	47.2