Hotspot

rps15-trnN-GUU

SSC · 122218-128202 · 5985 bp

Back to Saussurea

Pi 0.00556

MarkerSeek score 59.10

Primer available yes

Label rps15-trnN-GUU

Feature Metadata

label_name: rps15-trnN-GUU
feature_type: igs
region: SSC
length_bp: 5985
variable_sites: 223
pi: 0.00556
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-trnN-GUU_p1	TTGCATTTCTTCTTCCGCCT	ATCCTTTTGGTCCTCCTCGA	45.0	50.0	58.1	58.0	341 bp	1.0	84.5
rps15-trnN-GUU_p2	TTGCATTTCTTCTTCCGCCT	CTAGGAAATGCCCATTCCCC	45.0	55.0	58.1	58.0	903 bp	1.0	83.1
rps15-trnN-GUU_p3	AGTTGTAGAGCGATTCTGCG	ATCCTTTTGGTCCTCCTCGA	50.0	50.0	58.1	58.0	577 bp	0.958333	80.6
rps15-trnN-GUU_p4	AGTTGTAGAGCGATTCTGCG	CTAGGAAATGCCCATTCCCC	50.0	55.0	58.1	58.0	1139 bp	0.958333	80.3
rps15-trnN-GUU_p5	AGTTGTAGAGCGATTCTGCG	CCAGATTTTCGTCGATCCGT	50.0	50.0	58.1	58.1	1214 bp	0.958333	79.7