Hotspot

rpl32-trnL-UAG

SSC · 112133-112996 · 864 bp

Back to Saussurea

Pi 0.014

MarkerSeek score 73.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 864
variable_sites: 65
pi: 0.014
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAGGAAAGGATATTGGGCGG	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.6	58.0	936-1016 bp	1.0	86.4
rpl32-trnL-UAG_p2	AAGGAAAGGATATTGGGCGG	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.6	58.0	937-1017 bp	1.0	86.4
rpl32-trnL-UAG_p3	CGGCGTTAAAAGCTTTGTCA	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.6	58.0	919-999 bp	1.0	86.2
rpl32-trnL-UAG_p4	CGGCGTTAAAAGCTTTGTCA	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.6	58.0	920-1000 bp	1.0	86.2
rpl32-trnL-UAG_p5	AAGGAAAGGATATTGGGCGG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.6	57.9	946-1026 bp	1.0	85.4