Hotspot

petA-psbJ

LSC · 62394-63214 · 821 bp

Back to Saussurea

Pi 0.00464

MarkerSeek score 68.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 821
variable_sites: 34
pi: 0.00464
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTACAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.0	57.6	924-990 bp	1.0	83.8
petA-psbJ_p2	ACGTGTACAAGGCCTTTTGT	TGTGATCGGTTTAGTAGGCG	45.0	50.0	58.0	57.4	962-1028 bp	1.0	81.9
petA-psbJ_p3	ACGTGTACAAGGCCTTTTGT	GTGATCGGTTTAGTAGGCGT	45.0	50.0	58.0	57.4	961-1027 bp	1.0	81.9
petA-psbJ_p4	GGCCTTTTGTTCTTCTTCGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.9	57.6	914-980 bp	0.958333	81.7
petA-psbJ_p5	GGCCTTTTGTTCTTCTTCGC	TGTGATCGGTTTAGTAGGCG	50.0	50.0	57.9	57.4	952-1018 bp	0.958333	79.9