Hotspot

ndhC-trnM-CAU

LSC · 49563-51547 · 1985 bp

Back to Saussurea

Pi 0.00396
MarkerSeek score 64.50
Primer available yes
Label ndhC-trnM-CAU

Feature Metadata

label_name
ndhC-trnM-CAU
feature_type
igs
region
LSC
length_bp
1985
variable_sites
55
pi
0.00396
unique_haplotype_count
23
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnM-CAU_p1 GTCCTTTGCTAATCGGAGCT CCCGCCGTATGAAAGCAATA 50.0 50.0 57.7 58.4 1888-2153 bp 1.0 80.3
ndhC-trnM-CAU_p2 GTCCTTTGCTAATCGGAGCT ATACCGCGGTCAATGGTATC 50.0 50.0 57.7 57.5 1947-2212 bp 0.958333 77.8
ndhC-trnM-CAU_p3 GTCCTTTGCTAATCGGAGCT CCGCGGTCAATGGTATCTAA 50.0 50.0 57.7 57.5 1944-2209 bp 0.958333 77.2
ndhC-trnM-CAU_p4 GTCCTTTGCTAATCGGAGCT TTAGATACCGCGGTCAATGG 50.0 50.0 57.7 57.5 1951-2216 bp 0.958333 77.2
ndhC-trnM-CAU_p5 CGGTCCTTTGCTAATCGGAG CCCGCCGTATGAAAGCAATA 55.0 50.0 58.4 58.4 1890-2155 bp 0.958333 77.2