Hotspot

trnT-UGU-trnF-GAA

LSC · 45892-47327 · 1436 bp

Back to Saussurea

Pi 0.00446

MarkerSeek score 65.30

Primer available yes

Label trnT-UGU-trnF-GAA

Feature Metadata

label_name: trnT-UGU-trnF-GAA
feature_type: igs
region: LSC
length_bp: 1436
variable_sites: 45
pi: 0.00446
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnF-GAA_p1	GCGATGCTCTAACCTCTGAG	TTTGAACTGGTGACACGAGG	55.0	50.0	57.9	58.1	1493-1553 bp	1.0	83.9
trnT-UGU-trnF-GAA_p2	GGAATCGAACCGATGACCAT	TTTGAACTGGTGACACGAGG	50.0	50.0	57.8	58.1	1525-1585 bp	1.0	83.0
trnT-UGU-trnF-GAA_p3	GCGATGCTCTAACCTCTGAG	TACCAACTGAGCTATCCCGA	55.0	50.0	57.9	57.6	1455-1515 bp	1.0	80.9
trnT-UGU-trnF-GAA_p4	ATGCGATGCTCTAACCTCTG	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	1495-1555 bp	1.0	80.8
trnT-UGU-trnF-GAA_p5	TGCGATGCTCTAACCTCTGA	TTTGAACTGGTGACACGAGG	50.0	50.0	58.5	58.1	1494-1554 bp	1.0	80.8