Hotspot

trnS-GCU-trnC-GCA

LSC · 8480-9260 · 781 bp

Back to Saussurea

Pi 0.0115

MarkerSeek score 68.10

Primer available yes

Label trnS-GCU-trnC-GCA

Feature Metadata

label_name: trnS-GCU-trnC-GCA
feature_type: igs
region: LSC
length_bp: 781
variable_sites: 80
pi: 0.0115
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnC-GCA_p1	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	903-944 bp	1.0	89.0
trnS-GCU-trnC-GCA_p2	ACGGAAAGAGAGGGATTCGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	58.4	885-926 bp	1.0	87.0
trnS-GCU-trnC-GCA_p3	CTTTCGTCCACTCAGCCATC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.6	58.1	839-880 bp	1.0	85.7
trnS-GCU-trnC-GCA_p4	AACGGAAAGAGAGGGATTCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.3	58.1	904-945 bp	1.0	84.9
trnS-GCU-trnC-GCA_p5	ACGGAAAGAGAGGGATTCGA	ATTCGATTCGTGATGAGGCG	50.0	50.0	58.2	58.5	937-956 bp	0.375	51.7