primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU_p1	trnK-UUU	1	CCAAGCGCACGAATTTTCAA	GGGTTCGTCGGATTAGCTTT	20	20	45.0	50.0	58.2	58.0	11.5	0.0	0.255097	1741	4272	2955-2976	1.000000	81.0
trnK-UUU_p2	trnK-UUU	2	GCCCGTGTCAACCAATATCT	GGGTTCGTCGGATTAGCTTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.058335	1741	4272	2888-2909	0.916667	79.2
trnK-UUU_p3	trnK-UUU	3	TGCCCGTGTCAACCAATATC	GGGTTCGTCGGATTAGCTTT	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.274716	1741	4272	2889-2910	0.916667	77.4
trnK-UUU_p4	trnK-UUU	4	GCCCGTGTCAACCAATATCT	CAAATGATGGGTTCGTCGGA	20	20	50.0	50.0	58.0	58.3	0.0	0.0	0.305446	1741	4272	2896-2917	0.875000	75.3
trnK-UUU_p5	trnK-UUU	5	GCCCGTGTCAACCAATATCT	TCAAATGATGGGTTCGTCGG	20	20	50.0	50.0	58.0	58.3	0.0	0.0	0.305446	1741	4272	2897-2918	0.875000	75.3
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6191	7139	1021-1047	1.000000	83.0
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	7.8	16.9	0.445953	6191	7139	1036-1062	1.000000	81.1
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	6191	7139	1035-1061	1.000000	81.1
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CCAAGAGCACCTTCATTCCT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.5	58.1	0.0	16.9	0.579996	6191	7139	1092-1118	1.000000	79.9
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6191	7139	1025-1051	1.000000	78.3
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	CGTAATCCTGGACGTGAAGA	ATTGGGAGAGATGGCTGAGT	20	20	50.0	50.0	57.1	58.1	0.0	0.0	1.042943	8256	8391	251-258	1.000000	78.3
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	CGTAATCCTGGACGTGAAGA	CGAGTTATTCGTACCGAGGG	20	20	50.0	55.0	57.1	57.6	0.0	0.0	1.329655	8256	8391	199-206	1.000000	77.7
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	CGTAATCCTGGACGTGAAGAA	ATTGGGAGAGATGGCTGAGT	21	20	47.6	50.0	57.8	58.1	0.0	0.0	1.335793	8256	8391	251-258	1.000000	75.9
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	CCTATCCAATGATCCAGGACG	ATTGGGAGAGATGGCTGAGT	21	20	52.4	50.0	57.7	58.1	0.0	0.0	1.434740	8256	8391	270-277	1.000000	74.7
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	CGTAATCCTGGACGTGAAGA	GATGGCTGAGTGGACGAAAG	20	20	50.0	55.0	57.1	58.6	0.0	0.0	1.569752	8256	8391	242-249	1.000000	74.3
trnS-GCU-trnC-GCA_p1	trnS-GCU-trnC-GCA	1	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.233476	8480	9260	903-944	1.000000	89.0
trnS-GCU-trnC-GCA_p2	trnS-GCU-trnC-GCA	2	ACGGAAAGAGAGGGATTCGA	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.2	58.4	0.0	14.6	0.506839	8480	9260	885-926	1.000000	87.0
trnS-GCU-trnC-GCA_p3	trnS-GCU-trnC-GCA	3	CTTTCGTCCACTCAGCCATC	CCGGATTTGAACTGGGGAAA	20	20	55.0	50.0	58.6	58.1	0.0	0.0	0.718384	8480	9260	839-880	1.000000	85.7
trnS-GCU-trnC-GCA_p4	trnS-GCU-trnC-GCA	4	AACGGAAAGAGAGGGATTCG	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.3	58.1	0.0	0.0	0.756923	8480	9260	904-945	1.000000	84.9
trnS-GCU-trnC-GCA_p5	trnS-GCU-trnC-GCA	5	ACGGAAAGAGAGGGATTCGA	ATTCGATTCGTGATGAGGCG	20	20	50.0	50.0	58.2	58.5	0.0	5.9	0.659678	8480	9260	937-956	0.375000	51.7
trnE-UUC-rpoB_p1	trnE-UUC-rpoB	1	GCATGATTTACCCCCAGAGG	CATTGCGTCTATCCCGGATT	20	20	55.0	50.0	58.0	57.8	0.0	0.7	0.182950	11565	12349	903-983	1.000000	85.3
trnE-UUC-rpoB_p2	trnE-UUC-rpoB	2	GGCATGATTTACCCCCAGAG	CATTGCGTCTATCCCGGATT	20	20	55.0	50.0	58.0	57.8	0.0	0.7	0.182950	11565	12349	904-984	1.000000	85.3
trnE-UUC-rpoB_p3	trnE-UUC-rpoB	3	ACCCTCGTTATCTCCGTGAA	CATTGCGTCTATCCCGGATT	20	20	50.0	50.0	58.2	57.8	0.0	0.7	0.326464	11565	12349	876-956	1.000000	84.2
trnE-UUC-rpoB_p4	trnE-UUC-rpoB	4	GCATGATTTACCCCCAGAGG	TGTGGACATTGCGTCTATCC	20	20	55.0	50.0	58.0	57.7	0.0	0.0	0.322645	11565	12349	909-989	1.000000	84.1
trnE-UUC-rpoB_p5	trnE-UUC-rpoB	5	GGCATGATTTACCCCCAGAG	TGTGGACATTGCGTCTATCC	20	20	55.0	50.0	58.0	57.7	0.0	0.0	0.322645	11565	12349	910-990	1.000000	84.1
atpI-atpH_p1	atpI-atpH	1	TCCCTGTCATGTTCCTTGGA	GGCAGAAACCAGTGGATTCA	20	20	50.0	50.0	58.3	58.1	0.0	28.1	0.349272	24581	25700	1232-1302	1.000000	81.6
atpI-atpH_p2	atpI-atpH	2	TCCCTGTCATGTTCCTTGGA	TACCTTGACCAACTCCAGGT	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.371251	24581	25700	1299-1369	1.000000	81.3
atpI-atpH_p3	atpI-atpH	3	TCCCTGTCATGTTCCTTGGA	AGCCAATCCAGCAGCAATAA	20	20	50.0	45.0	58.3	57.9	0.0	0.0	0.407418	24581	25700	1259-1329	1.000000	81.1
atpI-atpH_p4	atpI-atpH	4	TCCCTGTCATGTTCCTTGGA	AATAACGGAAGCGGCAGAAA	20	20	50.0	45.0	58.3	58.2	0.0	1.4	0.442898	24581	25700	1244-1314	1.000000	80.8
atpI-atpH_p5	atpI-atpH	5	TCCCTGTCATGTTCCTTGGA	GCAGTACCTTGACCAACTCC	20	20	50.0	55.0	58.3	58.2	0.0	0.0	0.449288	24581	25700	1303-1373	0.958333	78.9
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	21	20	47.6	50.0	58.0	58.2	8.2	0.0	1.191833	43550	44470	972-1082	1.000000	78.4
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTCAA	21	20	47.6	45.0	58.0	57.8	8.2	0.0	1.233148	43550	44470	922-1032	1.000000	78.2
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	CATTTATCCGCGATCTAGGCA	GGATTCGAACCCTCGGTAAG	21	20	47.6	55.0	58.0	57.8	8.2	0.0	1.260963	43550	44470	960-1070	1.000000	77.9
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTC	21	20	47.6	50.0	58.0	58.3	8.2	0.0	1.301418	43550	44470	924-1034	1.000000	77.7
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	CATTTATCCGCGATCTAGGCA	CCTCGGTAAGCAAAAGCCTA	21	20	47.6	50.0	58.0	57.6	8.2	0.0	1.437074	43550	44470	950-1060	1.000000	76.5
trnT-UGU-trnF-GAA_p1	trnT-UGU-trnF-GAA	1	GCGATGCTCTAACCTCTGAG	TTTGAACTGGTGACACGAGG	20	20	55.0	50.0	57.9	58.1	0.0	20.1	0.184636	45892	47327	1493-1553	1.000000	83.9
trnT-UGU-trnF-GAA_p2	trnT-UGU-trnF-GAA	2	GGAATCGAACCGATGACCAT	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.8	58.1	15.5	20.1	0.292389	45892	47327	1525-1585	1.000000	83.0
trnT-UGU-trnF-GAA_p3	trnT-UGU-trnF-GAA	3	GCGATGCTCTAACCTCTGAG	TACCAACTGAGCTATCCCGA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.580148	45892	47327	1455-1515	1.000000	80.9
trnT-UGU-trnF-GAA_p4	trnT-UGU-trnF-GAA	4	ATGCGATGCTCTAACCTCTG	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.5	58.1	0.0	20.1	0.574731	45892	47327	1495-1555	1.000000	80.8
trnT-UGU-trnF-GAA_p5	trnT-UGU-trnF-GAA	5	TGCGATGCTCTAACCTCTGA	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	58.5	58.1	0.0	20.1	0.577566	45892	47327	1494-1554	1.000000	80.8
ndhC-trnM-CAU_p1	ndhC-trnM-CAU	1	GTCCTTTGCTAATCGGAGCT	CCCGCCGTATGAAAGCAATA	20	20	50.0	50.0	57.7	58.4	0.0	0.0	0.728856	49563	51547	1888-2153	1.000000	80.3
ndhC-trnM-CAU_p2	ndhC-trnM-CAU	2	GTCCTTTGCTAATCGGAGCT	ATACCGCGGTCAATGGTATC	20	20	50.0	50.0	57.7	57.5	0.0	38.0	0.768139	49563	51547	1947-2212	0.958333	77.8
ndhC-trnM-CAU_p3	ndhC-trnM-CAU	3	GTCCTTTGCTAATCGGAGCT	CCGCGGTCAATGGTATCTAA	20	20	50.0	50.0	57.7	57.5	0.0	19.5	0.836906	49563	51547	1944-2209	0.958333	77.2
ndhC-trnM-CAU_p4	ndhC-trnM-CAU	4	GTCCTTTGCTAATCGGAGCT	TTAGATACCGCGGTCAATGG	20	20	50.0	50.0	57.7	57.5	0.0	38.0	0.836906	49563	51547	1951-2216	0.958333	77.2
ndhC-trnM-CAU_p5	ndhC-trnM-CAU	5	CGGTCCTTTGCTAATCGGAG	CCCGCCGTATGAAAGCAATA	20	20	55.0	50.0	58.4	58.4	0.0	0.0	0.839407	49563	51547	1890-2155	0.958333	77.2
atpB-rbcL_p1	atpB-rbcL	1	CTGGCGGAAAAGCTACATCT	CACCAGCTTTGAATCCAACAC	20	21	50.0	47.6	58.0	58.0	0.0	0.0	1.047103	53715	54472	887-940	1.000000	76.9
atpB-rbcL_p2	atpB-rbcL	2	CTGGCGGAAAAGCTACATCT	ACCAGCTTTGAATCCAACACT	20	21	50.0	42.9	58.0	58.1	0.0	0.0	1.080391	53715	54472	886-939	1.000000	76.7
atpB-rbcL_p3	atpB-rbcL	3	GATACGCCCCAGGTTCTTTT	CACCAGCTTTGAATCCAACAC	20	21	50.0	47.6	57.9	58.0	0.0	0.0	1.141542	53715	54472	844-897	1.000000	76.2
atpB-rbcL_p4	atpB-rbcL	4	GGAAACCCCAGAACCAGAAG	CACCAGCTTTGAATCCAACAC	20	21	55.0	47.6	58.1	58.0	0.0	0.0	1.106208	53715	54472	827-867	0.958333	73.6
atpB-rbcL_p5	atpB-rbcL	5	GGAAACCCCAGAACCAGAAG	ACCAGCTTTGAATCCAACACT	20	21	55.0	42.9	58.1	58.1	0.0	0.0	1.139495	53715	54472	826-866	0.958333	73.3
rbcL_p1	rbcL	1	TCATGAGTTGTAGGGAGGGA	GGGTTCGGCTCAATCCTTTT	20	20	50.0	50.0	57.1	58.5	0.0	0.0	1.394673	54473	55900	1565-1565	1.000000	70.3
rbcL_p2	rbcL	2	ACGCCTAATTCATGTCGAGT	GGGTTCGGCTCAATCCTTTT	20	20	45.0	50.0	57.0	58.5	0.0	0.0	1.415685	54473	55900	1613-1613	1.000000	70.1
rbcL_p3	rbcL	3	TCATGAGTTGTAGGGAGGGA	ATGTTGGGTTCGGCTCAATC	20	20	50.0	50.0	57.1	58.5	0.0	0.0	1.476840	54473	55900	1570-1570	1.000000	69.6
rbcL_p4	rbcL	4	ACGCCTAATTCATGTCGAGT	ATGTTGGGTTCGGCTCAATC	20	20	45.0	50.0	57.0	58.5	0.0	0.0	1.497852	54473	55900	1618-1618	1.000000	69.4
rbcL_p5	rbcL	5	TTCATGAGTTGTAGGGAGGGA	GGGTTCGGCTCAATCCTTTT	21	20	47.6	50.0	57.8	58.5	0.0	0.0	1.653601	54473	55900	1566-1566	1.000000	68.2
petA-psbJ_p1	petA-psbJ	1	ACGTGTACAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	58.0	57.6	17.6	4.1	0.411974	62394	63214	924-990	1.000000	83.8
petA-psbJ_p2	petA-psbJ	2	ACGTGTACAAGGCCTTTTGT	TGTGATCGGTTTAGTAGGCG	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.619192	62394	63214	962-1028	1.000000	81.9
petA-psbJ_p3	petA-psbJ	3	ACGTGTACAAGGCCTTTTGT	GTGATCGGTTTAGTAGGCGT	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.620943	62394	63214	961-1027	1.000000	81.9
petA-psbJ_p4	petA-psbJ	4	GGCCTTTTGTTCTTCTTCGC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.9	57.6	0.0	4.1	0.416851	62394	63214	914-980	0.958333	81.7
petA-psbJ_p5	petA-psbJ	5	GGCCTTTTGTTCTTCTTCGC	TGTGATCGGTTTAGTAGGCG	20	20	50.0	50.0	57.9	57.4	0.0	0.0	0.624069	62394	63214	952-1018	0.958333	79.9
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.9	57.1	6.4	0.0	0.992787	64006	65292	1442-1489	1.000000	78.7
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	24.9	0.0	1.097796	64006	65292	1365-1412	1.000000	78.3
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	20	20	45.0	50.0	58.2	57.1	0.0	0.0	1.112474	64006	65292	1451-1498	1.000000	77.7
psbE-petL_p4	psbE-petL	4	TGCTGACGAATAACCAACCC	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	58.2	57.1	0.0	0.0	1.125378	64006	65292	1456-1503	1.000000	77.6
psbE-petL_p5	psbE-petL	5	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	0.0	0.0	1.128804	64006	65292	1450-1497	1.000000	77.6
ndhF-rpl32_p1	ndhF-rpl32	1	GTTGCCGTCGGAAAAAGAAG	CCGCCCAATATCCTTTCCTT	20	20	50.0	50.0	58.0	57.6	17.5	0.0	0.397386	110918	111967	1225-1238	1.000000	80.7
ndhF-rpl32_p2	ndhF-rpl32	2	TGCCGTCGGAAAAAGAAGAA	CCGCCCAATATCCTTTCCTT	20	20	45.0	50.0	58.0	57.6	17.5	0.0	0.408676	110918	111967	1223-1236	1.000000	80.6
ndhF-rpl32_p3	ndhF-rpl32	3	TTGCCGTCGGAAAAAGAAGA	CCGCCCAATATCCTTTCCTT	20	20	45.0	50.0	58.0	57.6	17.5	0.0	0.408676	110918	111967	1224-1237	1.000000	80.6
ndhF-rpl32_p4	ndhF-rpl32	4	GTTGCCGTCGGAAAAAGAAG	TGACAAAGCTTTTAACGCCG	20	20	50.0	45.0	58.0	57.6	17.5	9.5	0.425347	110918	111967	1242-1255	1.000000	80.4
ndhF-rpl32_p5	ndhF-rpl32	5	TGCCGTCGGAAAAAGAAGAA	TGACAAAGCTTTTAACGCCG	20	20	45.0	45.0	58.0	57.6	17.5	9.5	0.436636	110918	111967	1240-1253	1.000000	80.3
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AAGGAAAGGATATTGGGCGG	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.372803	112133	112996	936-1016	1.000000	86.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AAGGAAAGGATATTGGGCGG	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.374173	112133	112996	937-1017	1.000000	86.4
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	CGGCGTTAAAAGCTTTGTCA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.6	58.0	13.5	0.0	0.400763	112133	112996	919-999	1.000000	86.2
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	CGGCGTTAAAAGCTTTGTCA	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.6	58.0	13.5	0.0	0.402133	112133	112996	920-1000	1.000000	86.2
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AAGGAAAGGATATTGGGCGG	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	57.6	57.9	0.0	0.0	0.489250	112133	112996	946-1026	1.000000	85.4
rps15-trnN-GUU_p1	rps15-trnN-GUU	1	TTGCATTTCTTCTTCCGCCT	ATCCTTTTGGTCCTCCTCGA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.137679	125159	125278	341-341	1.000000	84.5
rps15-trnN-GUU_p2	rps15-trnN-GUU	2	TTGCATTTCTTCTTCCGCCT	CTAGGAAATGCCCATTCCCC	20	20	45.0	55.0	58.1	58.0	0.0	8.2	0.091376	125159	125278	903-903	1.000000	83.1
rps15-trnN-GUU_p3	rps15-trnN-GUU	3	AGTTGTAGAGCGATTCTGCG	ATCCTTTTGGTCCTCCTCGA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.132745	125159	125278	577-577	0.958333	80.6
rps15-trnN-GUU_p4	rps15-trnN-GUU	4	AGTTGTAGAGCGATTCTGCG	CTAGGAAATGCCCATTCCCC	20	20	50.0	55.0	58.1	58.0	0.0	8.2	0.086442	125159	125278	1139-1139	0.958333	80.3
rps15-trnN-GUU_p5	rps15-trnN-GUU	5	AGTTGTAGAGCGATTCTGCG	CCAGATTTTCGTCGATCCGT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.162796	125159	125278	1214-1214	0.958333	79.7