Hotspot

ycf1

SSC · 130831-136332 · 5502 bp

Back to Saururus

Pi 0.326

MarkerSeek score 75.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5502
variable_sites: 1585
pi: 0.326
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTTCGGGGAGAACCAAAGG	CCGATTGTGCCATGATGAGA	50.0	50.0	58.0	58.0	1345 bp	0.5	59.6
ycf1_p2	TTTTCGGGGAGAACCAAAGG	TCCGATTGTGCCATGATGAG	50.0	50.0	58.0	58.0	1346 bp	0.5	59.6
ycf1_p3	CCGCTATTGTTACCCGTTCA	CCGATTGTGCCATGATGAGA	50.0	50.0	58.0	58.0	929 bp	0.5	59.6
ycf1_p4	CCGCTATTGTTACCCGTTCA	TCCGATTGTGCCATGATGAG	50.0	50.0	58.0	58.0	930 bp	0.5	59.6
ycf1_p5	CCGTACGGGTTGAGCAAATA	CCGATTGTGCCATGATGAGA	50.0	50.0	58.0	58.0	967 bp	0.5	59.6