Hotspot

rpl32

SSC · 119663-119836 · 174 bp

Back to Saururus

Pi 0.46
MarkerSeek score 77.00
Primer available yes
Label rpl32

Feature Metadata

label_name
rpl32
feature_type
gene
region
SSC
length_bp
174
variable_sites
75
pi
0.46
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32_p1 TGAGGTGGAGGACGAATTCT GGAACAGATCCGTGGTATGG 50.0 55.0 58.1 57.8 965 bp 0.5 57.6
rpl32_p2 TGAGGTGGAGGACGAATTCT TCGAGTCATGTCAATTGCGA 50.0 45.0 58.1 57.6 488 bp 0.5 56.6
rpl32_p3 TACTCTATCCTCGGGAGTGC GGAACAGATCCGTGGTATGG 55.0 55.0 57.7 57.8 1221 bp 0.5 55.9
rpl32_p4 CTCTATCCTCGGGAGTGCTT GGAACAGATCCGTGGTATGG 55.0 55.0 58.3 57.8 1219 bp 0.5 55.7
rpl32_p5 TACTCTATCCTCGGGAGTGC TCGAGTCATGTCAATTGCGA 55.0 45.0 57.7 57.6 744 bp 0.5 54.9