Hotspot

ndhH

SSC · 128885-130066 · 1182 bp

Back to Saururus

Pi 0.502

MarkerSeek score 79.40

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 473
pi: 0.502
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GGTTTCATTCATCGACCCGA	AGGGGTCTGCGGAAAATTTT	50.0	45.0	58.0	58.0	2093 bp	0.5	59.8
ndhH_p2	AGGTTTCATTCATCGACCCG	AGGGGTCTGCGGAAAATTTT	50.0	45.0	58.0	58.0	2094 bp	0.5	59.7
ndhH_p3	ATACGTTGTTGTATCCCCGC	AGGGGTCTGCGGAAAATTTT	50.0	45.0	58.1	58.0	1609 bp	0.5	59.5
ndhH_p4	GGTTTCATTCATCGACCCGA	CCCAAACCCCAAAACTGGTA	50.0	50.0	58.0	57.9	2330 bp	0.5	59.1
ndhH_p5	GGTTTCATTCATCGACCCGA	GTCAACGGTTGCTGGACTAT	50.0	50.0	58.0	57.9	2065 bp	0.5	59.1