Hotspot

ndhF

SSC · 116177-118396 · 2220 bp

Back to Saururus

Pi 0.465

MarkerSeek score 77.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 807
pi: 0.465
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CCGATTGTGCCATGATGAGA	CCGTTGGGTAGAGTTTTCGT	50.0	50.0	58.0	57.8	2798 bp	0.5	58.4
ndhF_p2	TCCGATTGTGCCATGATGAG	CCGTTGGGTAGAGTTTTCGT	50.0	50.0	58.0	57.8	2799 bp	0.5	58.4
ndhF_p3	CAGAAAAAGGATCCCCGGAG	CCGTTGGGTAGAGTTTTCGT	55.0	50.0	58.0	57.8	2943 bp	0.5	58.4
ndhF_p4	GGATCCGATTGTGCCATGAT	CCGTTGGGTAGAGTTTTCGT	50.0	50.0	58.1	57.8	2802 bp	0.5	57.9
ndhF_p5	GACAGAAAAAGGATCCCCGG	CCGTTGGGTAGAGTTTTCGT	55.0	50.0	58.2	57.8	2945 bp	0.5	56.7