Hotspot

ndhA

SSC · 126683-128883 · 2201 bp

Back to Saururus

Pi 0.469

MarkerSeek score 79.90

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2201
variable_sites: 900
pi: 0.469
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GGTAAACGATTCGTGTGGGA	CAATGTTACGAGCTTCCGGA	50.0	50.0	57.9	58.0	2913 bp	0.5	59.3
ndhA_p2	AGGTAAACGATTCGTGTGGG	CAATGTTACGAGCTTCCGGA	50.0	50.0	57.9	58.0	2914 bp	0.5	59.3
ndhA_p3	GGTAAACGATTCGTGTGGGA	TCGGTGAAATGACGGAATCC	50.0	50.0	57.9	58.0	2784 bp	0.5	59.2
ndhA_p4	AGGTAAACGATTCGTGTGGG	TCGGTGAAATGACGGAATCC	50.0	50.0	57.9	58.0	2785 bp	0.5	59.1
ndhA_p5	GGTAAACGATTCGTGTGGGA	TTTGGAACGGGTTGAAGGAG	50.0	50.0	57.9	58.0	2981 bp	0.5	59.1