Hotspot

rps15-ycf1

SSC · 130452-130830 · 379 bp

Back to Saururus

Pi 0.395

MarkerSeek score 77.10

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 379
variable_sites: 132
pi: 0.395
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AAAATTTTCCGCAGACCCCT	GGAGAAATGCGACTGGATGT	45.0	50.0	58.0	58.0	1149 bp	0.5	59.7
rps15-ycf1_p2	AACGAGTTTTTGGTTCCCGA	GGAGAAATGCGACTGGATGT	45.0	50.0	58.0	58.0	1250 bp	0.5	59.4
rps15-ycf1_p3	ACGAGTTTTTGGTTCCCGAA	GGAGAAATGCGACTGGATGT	45.0	50.0	58.0	58.0	1249 bp	0.5	59.4
rps15-ycf1_p4	TGCATTGATGGGTGATGAGG	GGAGAAATGCGACTGGATGT	50.0	50.0	57.9	58.0	1438 bp	0.5	59.3
rps15-ycf1_p5	ATGAGTCGAAGAACACCGTG	GGAGAAATGCGACTGGATGT	50.0	50.0	57.9	58.0	1456 bp	0.5	59.2