Hotspot

ccsA-ndhD

SSC · 122132-122404 · 273 bp

Back to Saururus

Pi 0.468

MarkerSeek score 76.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 273
variable_sites: 118
pi: 0.468
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GGGTGTGAATTCGGCAATTG	GTGGGAGCGGTCCAAATAAT	50.0	50.0	58.0	57.9	1057 bp	0.5	59.5
ccsA-ndhD_p2	AAGTGCAATTACAGCCCCAA	GTGGGAGCGGTCCAAATAAT	45.0	50.0	58.0	57.9	1612 bp	0.5	59.5
ccsA-ndhD_p3	GGGTGTGAATTCGGCAATTG	TTGCATTGCCAGGAATGAGT	50.0	45.0	58.0	58.1	747 bp	0.5	59.4
ccsA-ndhD_p4	AAGTGCAATTACAGCCCCAA	TTGCATTGCCAGGAATGAGT	45.0	45.0	58.0	58.1	1302 bp	0.5	59.4
ccsA-ndhD_p5	GGGTGTGAATTCGGCAATTG	ATTATAGTGGGAGCGGTCCA	50.0	50.0	58.0	57.9	1063 bp	0.5	59.2