Hotspot

rpl32-trnL-UAG

SSC · 119837-120951 · 1115 bp

Back to Saururus

Pi 0.428

MarkerSeek score 78.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1115
variable_sites: 360
pi: 0.428
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGAGGTGGAGGACGAATTCT	TTGGGGCTGTAATTGCACTT	50.0	45.0	58.1	58.0	2104 bp	0.5	59.4
rpl32-trnL-UAG_p2	TGAGGTGGAGGACGAATTCT	TCGTGCCCTTTTCTAACGAG	50.0	50.0	58.1	57.9	1927 bp	0.5	58.9
rpl32-trnL-UAG_p3	TGAGGTGGAGGACGAATTCT	AGAAGTAGCTATCGTGCCCT	50.0	50.0	58.1	57.9	1938 bp	0.5	58.9
rpl32-trnL-UAG_p4	AAGGATATTGGGCGGCAAAA	TTGGGGCTGTAATTGCACTT	45.0	45.0	58.1	58.0	1743 bp	0.5	58.8
rpl32-trnL-UAG_p5	AGGATATTGGGCGGCAAAAA	TTGGGGCTGTAATTGCACTT	45.0	45.0	58.1	58.0	1742 bp	0.5	58.8