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psbT-psbN

LSC · 79166-79228 · 63 bp

Back to Saururus

Pi 0.0794

MarkerSeek score 81.80

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 63
variable_sites: 5
pi: 0.0794
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CGCCTAGGATTTCGACGAAA	AGATCCGTTCGAAGAACACG	50.0	50.0	58.1	58.0	123 bp	1.0	82.4
psbT-psbN_p2	CGCCTAGGATTTCGACGAAA	TCGCCATCTCCATATCTGGT	50.0	50.0	58.1	58.0	208 bp	1.0	81.1
psbT-psbN_p3	CGCCTAGGATTTCGACGAAA	CCCTAGTCGCCATCTCCATA	50.0	55.0	58.1	58.1	214 bp	1.0	80.6
psbT-psbN_p4	CGAGAACCGCCTAGGATTTC	TCGCCATCTCCATATCTGGT	55.0	50.0	58.1	58.0	215 bp	1.0	80.6
psbT-psbN_p5	GAGAACCGCCTAGGATTTCG	TCGCCATCTCCATATCTGGT	55.0	50.0	58.1	58.0	214 bp	1.0	80.6