primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score psbT-psbN_p1 psbT-psbN 1 CGCCTAGGATTTCGACGAAA AGATCCGTTCGAAGAACACG 20 20 50.0 50.0 58.1 58.0 24.0 12.6 0.104186 79166 79228 123-123 1.000000 82.4 psbT-psbN_p2 psbT-psbN 2 CGCCTAGGATTTCGACGAAA TCGCCATCTCCATATCTGGT 20 20 50.0 50.0 58.1 58.0 24.0 0.0 0.098184 79166 79228 208-208 1.000000 81.1 psbT-psbN_p3 psbT-psbN 3 CGCCTAGGATTTCGACGAAA CCCTAGTCGCCATCTCCATA 20 20 50.0 55.0 58.1 58.1 24.0 0.0 0.157996 79166 79228 214-214 1.000000 80.6 psbT-psbN_p4 psbT-psbN 4 CGAGAACCGCCTAGGATTTC TCGCCATCTCCATATCTGGT 20 20 55.0 50.0 58.1 58.0 14.8 0.0 0.161230 79166 79228 215-215 1.000000 80.6 psbT-psbN_p5 psbT-psbN 5 GAGAACCGCCTAGGATTTCG TCGCCATCTCCATATCTGGT 20 20 55.0 50.0 58.1 58.0 14.8 0.0 0.161230 79166 79228 214-214 1.000000 80.6 ndhF_p1 ndhF 1 CCGATTGTGCCATGATGAGA CCGTTGGGTAGAGTTTTCGT 20 20 50.0 50.0 58.0 57.8 0.0 0.0 0.201659 116177 118396 2798-2798 0.500000 58.4 ndhF_p2 ndhF 2 TCCGATTGTGCCATGATGAG CCGTTGGGTAGAGTTTTCGT 20 20 50.0 50.0 58.0 57.8 0.0 0.0 0.201659 116177 118396 2799-2799 0.500000 58.4 ndhF_p3 ndhF 3 CAGAAAAAGGATCCCCGGAG CCGTTGGGTAGAGTTTTCGT 20 20 55.0 50.0 58.0 57.8 8.6 0.0 0.203450 116177 118396 2943-2943 0.500000 58.4 ndhF_p4 ndhF 4 GGATCCGATTGTGCCATGAT CCGTTGGGTAGAGTTTTCGT 20 20 50.0 50.0 58.1 57.8 0.0 0.0 0.257736 116177 118396 2802-2802 0.500000 57.9 ndhF_p5 ndhF 5 GACAGAAAAAGGATCCCCGG CCGTTGGGTAGAGTTTTCGT 20 20 55.0 50.0 58.2 57.8 8.6 0.0 0.407890 116177 118396 2945-2945 0.500000 56.7 ndhF-rpl32_p1 ndhF-rpl32 1 AGTAACTGGGAGTGGAACGA TGCCGCCCAATATCCTTTTT 20 20 50.0 45.0 58.0 58.1 0.0 0.0 0.146068 118397 119662 1407-1407 0.500000 58.8 ndhF-rpl32_p2 ndhF-rpl32 2 AGTAACTGGGAGTGGAACGA TTGCCGCCCAATATCCTTTT 20 20 50.0 45.0 58.0 58.1 0.0 0.0 0.146068 118397 119662 1408-1408 0.500000 58.8 ndhF-rpl32_p3 ndhF-rpl32 3 AGTAACTGGGAGTGGAACGA TTTGCCGCCCAATATCCTTT 20 20 50.0 45.0 58.0 58.1 0.0 0.0 0.146068 118397 119662 1409-1409 0.500000 58.8 ndhF-rpl32_p4 ndhF-rpl32 4 AGTAACTGGGAGTGGAACGA TTTTGCCGCCCAATATCCTT 20 20 50.0 45.0 58.0 58.1 0.0 0.0 0.146068 118397 119662 1410-1410 0.500000 58.8 ndhF-rpl32_p5 ndhF-rpl32 5 AGTAACTGGGAGTGGAACGA TTTTTGCCGCCCAATATCCT 20 20 50.0 45.0 58.0 58.1 0.0 0.0 0.146068 118397 119662 1411-1411 0.500000 58.8 rpl32_p1 rpl32 1 TGAGGTGGAGGACGAATTCT GGAACAGATCCGTGGTATGG 20 20 50.0 55.0 58.1 57.8 3.5 0.0 0.301909 119663 119836 965-965 0.500000 57.6 rpl32_p2 rpl32 2 TGAGGTGGAGGACGAATTCT TCGAGTCATGTCAATTGCGA 20 20 50.0 45.0 58.1 57.6 3.5 0.0 0.423585 119663 119836 488-488 0.500000 56.6 rpl32_p3 rpl32 3 TACTCTATCCTCGGGAGTGC GGAACAGATCCGTGGTATGG 20 20 55.0 55.0 57.7 57.8 0.0 0.0 0.514031 119663 119836 1221-1221 0.500000 55.9 rpl32_p4 rpl32 4 CTCTATCCTCGGGAGTGCTT GGAACAGATCCGTGGTATGG 20 20 55.0 55.0 58.3 57.8 0.0 0.0 0.541987 119663 119836 1219-1219 0.500000 55.7 rpl32_p5 rpl32 5 TACTCTATCCTCGGGAGTGC TCGAGTCATGTCAATTGCGA 20 20 55.0 45.0 57.7 57.6 0.0 0.0 0.635707 119663 119836 744-744 0.500000 54.9 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 TGAGGTGGAGGACGAATTCT TTGGGGCTGTAATTGCACTT 20 20 50.0 45.0 58.1 58.0 3.5 0.0 0.069658 119837 120951 2104-2104 0.500000 59.4 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 TGAGGTGGAGGACGAATTCT TCGTGCCCTTTTCTAACGAG 20 20 50.0 50.0 58.1 57.9 3.5 0.0 0.135962 119837 120951 1927-1927 0.500000 58.9 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 TGAGGTGGAGGACGAATTCT AGAAGTAGCTATCGTGCCCT 20 20 50.0 50.0 58.1 57.9 3.5 0.0 0.136144 119837 120951 1938-1938 0.500000 58.9 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 AAGGATATTGGGCGGCAAAA TTGGGGCTGTAATTGCACTT 20 20 45.0 45.0 58.1 58.0 12.5 0.0 0.148941 119837 120951 1743-1743 0.500000 58.8 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 AGGATATTGGGCGGCAAAAA TTGGGGCTGTAATTGCACTT 20 20 45.0 45.0 58.1 58.0 12.5 0.0 0.148941 119837 120951 1742-1742 0.500000 58.8 trnL-UAG-ccsA_p1 trnL-UAG-ccsA 1 GCCTAATCGTCCCGGATTTT TTGGGGCTGTAATTGCACTT 20 20 50.0 45.0 58.0 58.0 0.7 0.0 0.045070 121032 121159 730-730 0.500000 59.6 trnL-UAG-ccsA_p2 trnL-UAG-ccsA 2 GCCTAATCGTCCCGGATTTT TACTGTACCCCGCCAAGTAA 20 20 50.0 50.0 58.0 58.1 0.7 0.0 0.101029 121032 121159 992-992 0.500000 59.2 trnL-UAG-ccsA_p3 trnL-UAG-ccsA 3 GCCTAATCGTCCCGGATTTT TCGTGCCCTTTTCTAACGAG 20 20 50.0 50.0 58.0 57.9 0.7 0.0 0.111375 121032 121159 553-553 0.500000 59.1 trnL-UAG-ccsA_p4 trnL-UAG-ccsA 4 GCCTAATCGTCCCGGATTTT AGAAGTAGCTATCGTGCCCT 20 20 50.0 50.0 58.0 57.9 0.7 0.0 0.111557 121032 121159 564-564 0.500000 59.1 trnL-UAG-ccsA_p5 trnL-UAG-ccsA 5 TAGACACGCTGCTCTTAGGA TTGGGGCTGTAATTGCACTT 20 20 50.0 45.0 57.9 58.0 0.0 0.0 0.132520 121032 121159 502-502 0.500000 58.9 ccsA_p1 ccsA 1 GCCTAATCGTCCCGGATTTT TTGCATTGCCAGGAATGAGT 20 20 50.0 45.0 58.0 58.1 0.7 15.1 0.109341 121160 122131 2012-2012 0.500000 59.1 ccsA_p2 ccsA 2 GCCTAATCGTCCCGGATTTT TTTGTAGATTCTGGGCCACG 20 20 50.0 50.0 58.0 57.9 0.7 0.0 0.144778 121160 122131 1809-1809 0.500000 58.8 ccsA_p3 ccsA 3 GCCTAATCGTCCCGGATTTT CATTGCCAGGAATGAGTGGT 20 20 50.0 50.0 58.0 58.2 0.7 6.0 0.193460 121160 122131 2009-2009 0.500000 58.5 ccsA_p4 ccsA 4 TAGACACGCTGCTCTTAGGA TTGCATTGCCAGGAATGAGT 20 20 50.0 45.0 57.9 58.1 0.0 15.1 0.196790 121160 122131 1784-1784 0.500000 58.4 ccsA_p5 ccsA 5 GCCTAATCGTCCCGGATTTT CTTCTCTTGCATTGCCAGGA 20 20 50.0 50.0 58.0 58.2 0.7 9.6 0.206549 121160 122131 2018-2018 0.500000 58.3 ccsA-ndhD_p1 ccsA-ndhD 1 GGGTGTGAATTCGGCAATTG GTGGGAGCGGTCCAAATAAT 20 20 50.0 50.0 58.0 57.9 0.0 32.9 0.057866 122132 122404 1057-1057 0.500000 59.5 ccsA-ndhD_p2 ccsA-ndhD 2 AAGTGCAATTACAGCCCCAA GTGGGAGCGGTCCAAATAAT 20 20 45.0 50.0 58.0 57.9 0.0 32.9 0.061025 122132 122404 1612-1612 0.500000 59.5 ccsA-ndhD_p3 ccsA-ndhD 3 GGGTGTGAATTCGGCAATTG TTGCATTGCCAGGAATGAGT 20 20 50.0 45.0 58.0 58.1 0.0 15.1 0.077597 122132 122404 747-747 0.500000 59.4 ccsA-ndhD_p4 ccsA-ndhD 4 AAGTGCAATTACAGCCCCAA TTGCATTGCCAGGAATGAGT 20 20 45.0 45.0 58.0 58.1 0.0 15.1 0.080755 122132 122404 1302-1302 0.500000 59.4 ccsA-ndhD_p5 ccsA-ndhD 5 GGGTGTGAATTCGGCAATTG ATTATAGTGGGAGCGGTCCA 20 20 50.0 50.0 58.0 57.9 0.0 32.9 0.102194 122132 122404 1063-1063 0.500000 59.2 ndhD_p1 ndhD 1 GGGTGTGAATTCGGCAATTG AGATGTGAATCCGCTTGTCC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.034922 122405 123910 2111-2111 0.500000 59.7 ndhD_p2 ndhD 2 GTTTGCAGGGTGTGAATTCG AGATGTGAATCCGCTTGTCC 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.080315 122405 123910 2118-2118 0.500000 59.4 ndhD_p3 ndhD 3 TTACTTGGCGGGGTACAGTA AGATGTGAATCCGCTTGTCC 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.094039 122405 123910 2404-2404 0.500000 59.2 ndhD_p4 ndhD 4 GGGTGTGAATTCGGCAATTG TCTTGGCCGTTTCTCATGAG 20 20 50.0 50.0 58.0 57.9 0.0 17.1 0.104225 122405 123910 2425-2425 0.500000 59.2 ndhD_p5 ndhD 5 AGGGTGTGAATTCGGCAATT AGATGTGAATCCGCTTGTCC 20 20 45.0 50.0 58.1 58.0 0.0 0.0 0.106483 122405 123910 2112-2112 0.500000 59.1 ndhG_p1 ndhG 1 CTCATGAGAAACGGCCAAGA AACTGCGTCGAGTATTGTCC 20 20 50.0 50.0 57.9 58.0 18.3 0.0 0.106972 125155 125685 1863-1863 0.500000 59.1 ndhG_p2 ndhG 2 CTCATGAGAAACGGCCAAGA CTGCGTCGAGTATTGTCCAA 20 20 50.0 50.0 57.9 58.0 18.3 0.0 0.108342 125155 125685 1861-1861 0.500000 59.1 ndhG_p3 ndhG 3 CTCATGAGAAACGGCCAAGA TTTTGTGGTAACTGCGTCGA 20 20 50.0 45.0 57.9 58.1 18.3 0.0 0.161863 125155 125685 1872-1872 0.500000 58.7 ndhG_p4 ndhG 4 ATCTGCTCATGAGAAACGGC AACTGCGTCGAGTATTGTCC 20 20 50.0 50.0 58.3 58.0 27.0 0.0 0.346489 125155 125685 1868-1868 0.500000 57.2 ndhG_p5 ndhG 5 ATCTGCTCATGAGAAACGGC CTGCGTCGAGTATTGTCCAA 20 20 50.0 50.0 58.3 58.0 27.0 0.0 0.347860 125155 125685 1866-1866 0.500000 57.2 ndhG-ndhI_p1 ndhG-ndhI 1 ATGAAGTAACCCCATCCCCA AACTGCGTCGAGTATTGTCC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.013432 125686 126086 894-894 0.500000 59.9 ndhG-ndhI_p2 ndhG-ndhI 2 ATGAAGTAACCCCATCCCCA CTGCGTCGAGTATTGTCCAA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.014802 125686 126086 892-892 0.500000 59.9 ndhG-ndhI_p3 ndhG-ndhI 3 CGTTTATGGCTCCCACGTAA AACTGCGTCGAGTATTGTCC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.020767 125686 126086 804-804 0.500000 59.8 ndhG-ndhI_p4 ndhG-ndhI 4 CGTTTATGGCTCCCACGTAA CTGCGTCGAGTATTGTCCAA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.022138 125686 126086 802-802 0.500000 59.8 ndhG-ndhI_p5 ndhG-ndhI 5 CCCAACGGTCCAAAGAGAAA AACTGCGTCGAGTATTGTCC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.032171 125686 126086 878-878 0.500000 59.7 ndhI_p1 ndhI 1 ATGAAGTAACCCCATCCCCA GCGTCAACCCATAGGCTTTA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.038282 126087 126599 1767-1767 0.500000 59.7 ndhI_p2 ndhI 2 CGTTTATGGCTCCCACGTAA GCGTCAACCCATAGGCTTTA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.045617 126087 126599 1677-1677 0.500000 59.6 ndhI_p3 ndhI 3 CCCAACGGTCCAAAGAGAAA GCGTCAACCCATAGGCTTTA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.057021 126087 126599 1751-1751 0.500000 59.5 ndhI_p4 ndhI 4 ATGAAGTAACCCCATCCCCA TGTACTTGGGAGGGTGGAAT 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.059348 126087 126599 1573-1573 0.500000 59.5 ndhI_p5 ndhI 5 CGTTTATGGCTCCCACGTAA TGTACTTGGGAGGGTGGAAT 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.066684 126087 126599 1483-1483 0.500000 59.5 ndhA_p1 ndhA 1 GGTAAACGATTCGTGTGGGA CAATGTTACGAGCTTCCGGA 20 20 50.0 50.0 57.9 58.0 13.2 0.0 0.087749 126683 128883 2913-2913 0.500000 59.3 ndhA_p2 ndhA 2 AGGTAAACGATTCGTGTGGG CAATGTTACGAGCTTCCGGA 20 20 50.0 50.0 57.9 58.0 13.2 0.0 0.093532 126683 128883 2914-2914 0.500000 59.3 ndhA_p3 ndhA 3 GGTAAACGATTCGTGTGGGA TCGGTGAAATGACGGAATCC 20 20 50.0 50.0 57.9 58.0 13.2 0.0 0.101708 126683 128883 2784-2784 0.500000 59.2 ndhA_p4 ndhA 4 AGGTAAACGATTCGTGTGGG TCGGTGAAATGACGGAATCC 20 20 50.0 50.0 57.9 58.0 13.2 0.0 0.107491 126683 128883 2785-2785 0.500000 59.1 ndhA_p5 ndhA 5 GGTAAACGATTCGTGTGGGA TTTGGAACGGGTTGAAGGAG 20 20 50.0 50.0 57.9 58.0 13.2 0.0 0.107507 126683 128883 2981-2981 0.500000 59.1 ndhH_p1 ndhH 1 GGTTTCATTCATCGACCCGA AGGGGTCTGCGGAAAATTTT 20 20 50.0 45.0 58.0 58.0 0.0 2.3 0.028223 128885 130066 2093-2093 0.500000 59.8 ndhH_p2 ndhH 2 AGGTTTCATTCATCGACCCG AGGGGTCTGCGGAAAATTTT 20 20 50.0 45.0 58.0 58.0 0.0 2.3 0.033857 128885 130066 2094-2094 0.500000 59.7 ndhH_p3 ndhH 3 ATACGTTGTTGTATCCCCGC AGGGGTCTGCGGAAAATTTT 20 20 50.0 45.0 58.1 58.0 0.0 2.3 0.065650 128885 130066 1609-1609 0.500000 59.5 ndhH_p4 ndhH 4 GGTTTCATTCATCGACCCGA CCCAAACCCCAAAACTGGTA 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.108142 128885 130066 2330-2330 0.500000 59.1 ndhH_p5 ndhH 5 GGTTTCATTCATCGACCCGA GTCAACGGTTGCTGGACTAT 20 20 50.0 50.0 58.0 57.9 0.0 15.9 0.113304 128885 130066 2065-2065 0.500000 59.1 rps15-ycf1_p1 rps15-ycf1 1 AAAATTTTCCGCAGACCCCT GGAGAAATGCGACTGGATGT 20 20 45.0 50.0 58.0 58.0 0.0 0.0 0.039520 130452 130830 1149-1149 0.500000 59.7 rps15-ycf1_p2 rps15-ycf1 2 AACGAGTTTTTGGTTCCCGA GGAGAAATGCGACTGGATGT 20 20 45.0 50.0 58.0 58.0 0.0 0.0 0.071089 130452 130830 1250-1250 0.500000 59.4 rps15-ycf1_p3 rps15-ycf1 3 ACGAGTTTTTGGTTCCCGAA GGAGAAATGCGACTGGATGT 20 20 45.0 50.0 58.0 58.0 0.0 0.0 0.071089 130452 130830 1249-1249 0.500000 59.4 rps15-ycf1_p4 rps15-ycf1 4 TGCATTGATGGGTGATGAGG GGAGAAATGCGACTGGATGT 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.091348 130452 130830 1438-1438 0.500000 59.3 rps15-ycf1_p5 rps15-ycf1 5 ATGAGTCGAAGAACACCGTG GGAGAAATGCGACTGGATGT 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.095831 130452 130830 1456-1456 0.500000 59.2 ycf1_p1 ycf1 1 TTTTCGGGGAGAACCAAAGG CCGATTGTGCCATGATGAGA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.052625 134232 134351 1345-1345 0.500000 59.6 ycf1_p2 ycf1 2 TTTTCGGGGAGAACCAAAGG TCCGATTGTGCCATGATGAG 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.052625 134232 134351 1346-1346 0.500000 59.6 ycf1_p3 ycf1 3 CCGCTATTGTTACCCGTTCA CCGATTGTGCCATGATGAGA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.055269 134232 134351 929-929 0.500000 59.6 ycf1_p4 ycf1 4 CCGCTATTGTTACCCGTTCA TCCGATTGTGCCATGATGAG 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.055269 134232 134351 930-930 0.500000 59.6 ycf1_p5 ycf1 5 CCGTACGGGTTGAGCAAATA CCGATTGTGCCATGATGAGA 20 20 50.0 50.0 58.0 58.0 27.0 0.0 0.055269 134232 134351 967-967 0.500000 59.6