Hotspot

ycf1

SSC · 126304-133254 · 6951 bp

Back to Saurauia

Pi 0.00491

MarkerSeek score 55.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 6951
variable_sites: 34
pi: 0.00491
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTCCATTCGGAAGGTTGCAA	GAAACAACGAAAGAAGCGGG	45.0	50.0	58.0	58.0	221 bp	1.0	80.7
ycf1_p2	TCCATTCGGAAGGTTGCAAT	GAAACAACGAAAGAAGCGGG	45.0	50.0	57.8	58.0	220 bp	1.0	79.1
ycf1_p3	TTCCATTCGGAAGGTTGCAA	CGAAACAACGAAAGAAGCGG	45.0	50.0	58.0	58.3	222 bp	1.0	78.3
ycf1_p4	TTCCATTCGGAAGGTTGCAA	ACGAAACAACGAAAGAAGCG	45.0	45.0	58.0	57.6	223 bp	1.0	78.1
ycf1_p5	TCCATTCGGAAGGTTGCAAT	CGAAACAACGAAAGAAGCGG	45.0	50.0	57.8	58.3	221 bp	1.0	76.6