Hotspot

trnK (UUU)

LSC · 3582-3618 · 37 bp

Back to Saurauia

Pi 0.027

MarkerSeek score 63.90

Primer available yes

Label trnK (UUU)

Feature Metadata

label_name: trnK (UUU)
feature_type: tRNA
region: LSC
length_bp: 37
variable_sites: 1
pi: 0.027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK (UUU)_p1	CGAATCCCTTGCTTCATCCA	AGTTCAATGTTTTGATTTTCTCGT	50.0	29.2	58.0	56.1	165 bp	1.0	40.5
trnK (UUU)_p2	ACGAATCCCTTGCTTCATCC	AGTTCAATGTTTTGATTTTCTCGT	50.0	29.2	58.0	56.1	166 bp	1.0	40.5
trnK (UUU)_p3	ATGGACGAATCCCTTGCTTC	AGTTCAATGTTTTGATTTTCTCGT	50.0	29.2	58.0	56.1	170 bp	1.0	40.5
trnK (UUU)_p4	GGACGAATCCCTTGCTTCAT	AGTTCAATGTTTTGATTTTCTCGT	50.0	29.2	58.0	56.1	168 bp	1.0	40.5
trnK (UUU)_p5	TCAGGATCAGTCGTGGTCTT	AGTTCAATGTTTTGATTTTCTCGT	50.0	29.2	58.1	56.1	208 bp	1.0	40.4