Hotspot

rpoC2

LSC · 16716-20864 · 4149 bp

Back to Saurauia

Pi 0.00265

MarkerSeek score 55.40

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4149
variable_sites: 11
pi: 0.00265
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TGATTTTAAGTGTGGCCCGT	TCAGATCCTGGTAACTAAGT	45.0	40.0	57.7	52.3	256 bp	1.0	41.2
rpoC2_p2	TGATTTTAAGTGTGGCCCGT	ACCTATAATCAGATCCTGGTAACT	45.0	37.5	57.7	56.2	264 bp	1.0	41.2
rpoC2_p3	TGATTTTAAGTGTGGCCCGT	ACCTATAATCAGATCCTGGT	45.0	40.0	57.7	52.1	264 bp	1.0	41.2
rpoC2_p4	TGATTTTAAGTGTGGCCCGT	AACCTATAATCAGATCCTGGT	45.0	38.1	57.7	53.1	265 bp	1.0	41.2
rpoC2_p5	TGATTTTAAGTGTGGCCCGT	ATCAGATCCTGGTAACTAAGT	45.0	38.1	57.7	53.1	257 bp	1.0	41.2