Hotspot

psbA

LSC · 1-824 · 824 bp

Back to Saurauia

Pi 0.00364

MarkerSeek score 60.80

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 824
variable_sites: 3
pi: 0.00364
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GTGCTCAGCCTGGAATACAA	AATCTGGGAAGCAGCATCTG	50.0	50.0	57.9	57.9	286 bp	1.0	79.2
psbA_p2	GTGCTCAGCCTGGAATACAA	CAATCTGGGAAGCAGCATCT	50.0	50.0	57.9	57.9	287 bp	1.0	79.2
psbA_p3	GTTGTGCTCAGCCTGGAATA	AATCTGGGAAGCAGCATCTG	50.0	50.0	57.9	57.9	289 bp	1.0	79.2
psbA_p4	TTGTGCTCAGCCTGGAATAC	AATCTGGGAAGCAGCATCTG	50.0	50.0	57.9	57.9	288 bp	1.0	79.2
psbA_p5	TTGTGCTCAGCCTGGAATAC	CAATCTGGGAAGCAGCATCT	50.0	50.0	57.9	57.9	289 bp	1.0	79.2