Hotspot

petD

LSC · 78493-79738 · 1246 bp

Back to Saurauia

Pi 0.00243

MarkerSeek score 60.90

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1246
variable_sites: 3
pi: 0.00243
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	AGTTGAGGGGAATTCTCCGA	GGGAGAATTCACTTTGAAGCG	50.0	47.6	58.0	57.5	1345-1355 bp	1.0	68.6
petD_p2	TGAGGGGAATTCTCCGAAGA	GGGAGAATTCACTTTGAAGCG	50.0	47.6	57.8	57.5	1342-1352 bp	1.0	67.0
petD_p3	ATTTCCCTTCAACCCAGCAA	GGGAGAATTCACTTTGAAGCG	45.0	47.6	57.6	57.5	1391-1401 bp	1.0	65.7
petD_p4	TTTCCCTTCAACCCAGCAAT	GGGAGAATTCACTTTGAAGCG	45.0	47.6	57.6	57.5	1390-1400 bp	1.0	65.7
petD_p5	AGTTGAGGGGAATTCTCCGA	TTCCCTAGATACACACGCAT	50.0	45.0	58.0	56.0	1320-1330 bp	1.0	64.7