Hotspot

ndhF-rpl32

SSC · 114657-115627 · 971 bp

Back to Saurauia

Pi 0.00524
MarkerSeek score 63.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
971
variable_sites
5
pi
0.00524
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACCGGAAGTGGAACAAAAGG AGCTTTTAAGGCTGCCCAAT 50.0 45.0 58.3 58.1 1100-1117 bp 1.0 78.6
ndhF-rpl32_p2 CCGGAAGTGGAACAAAAGGT AGCTTTTAAGGCTGCCCAAT 50.0 45.0 58.3 58.1 1099-1116 bp 1.0 78.6
ndhF-rpl32_p3 GGAACCGGAAGTGGAACAAA AGCTTTTAAGGCTGCCCAAT 50.0 45.0 58.3 58.1 1103-1120 bp 1.0 78.6
ndhF-rpl32_p4 ACCGGAAGTGGAACAAAAGG AAGGCTGCCCAATATGCTTT 50.0 45.0 58.3 58.1 1093-1110 bp 1.0 78.1
ndhF-rpl32_p5 CCGGAAGTGGAACAAAAGGT AAGGCTGCCCAATATGCTTT 50.0 45.0 58.3 58.1 1092-1109 bp 1.0 78.1