Hotspot

rpl23-trnH (GUG)

IRb · 88215-89034 · 820 bp

Back to Saurauia

Pi 0.00366
MarkerSeek score 60.90
Primer available yes
Label rpl23-trnH (GUG)

Feature Metadata

label_name
rpl23-trnH (GUG)
feature_type
igs
region
IRb
length_bp
820
variable_sites
3
pi
0.00366
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23-trnH (GUG)_p1 CTGCATATTTGATGCCATCCA CTGCCTTAATCCACTTGGCT 42.9 50.0 56.7 57.9 871 bp 1.0 60.8
rpl23-trnH (GUG)_p2 ACTGCATATTTGATGCCATCC CTGCCTTAATCCACTTGGCT 42.9 50.0 56.7 57.9 872 bp 1.0 60.8
rpl23-trnH (GUG)_p3 CTGCATATTTGATGCCATCCA ACTGCCTTAATCCACTTGGC 42.9 50.0 56.7 58.2 872 bp 1.0 60.6
rpl23-trnH (GUG)_p4 ACTGCATATTTGATGCCATCC ACTGCCTTAATCCACTTGGC 42.9 50.0 56.7 58.2 873 bp 1.0 60.6
rpl23-trnH (GUG)_p5 ACTGCATATTTGATGCCATCCA CTGCCTTAATCCACTTGGCT 40.9 50.0 58.4 57.9 872 bp 1.0 59.7