Hotspot

rpl20-rps12

LSC · 71595-72363 · 769 bp

Back to Saurauia

Pi 0.0078

MarkerSeek score 57.90

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 769
variable_sites: 6
pi: 0.0078
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	AAGGCTTGAACGATTCCCTC	CGAAATCCCCTGCTCTTAGG	50.0	55.0	57.9	57.8	912-922 bp	1.0	77.6
rpl20-rps12_p2	TTCCCTCGACAGTTTGATGC	CGAAATCCCCTGCTCTTAGG	50.0	55.0	58.2	57.8	899-909 bp	1.0	77.0
rpl20-rps12_p3	CCTCGACAGTTTGATGCCAT	CGAAATCCCCTGCTCTTAGG	50.0	55.0	58.3	57.8	896-906 bp	1.0	76.5
rpl20-rps12_p4	AAGGCTTGAACGATTCCCTC	AATGTCACGAAATCCCCTGC	50.0	50.0	57.9	58.5	919-929 bp	1.0	75.3
rpl20-rps12_p5	TTCCCTCGACAGTTTGATGC	AATGTCACGAAATCCCCTGC	50.0	50.0	58.2	58.5	906-916 bp	1.0	74.7