Hotspot

ycf4-cemA

LSC · 62641-63564 · 924 bp

Back to Saurauia

Pi 0.00649

MarkerSeek score 62.50

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 924
variable_sites: 6
pi: 0.00649
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	TAGAGAAATCCACCAGGGCA	45.0	50.0	58.1	58.0	1038-1056 bp	1.0	79.4
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.1	58.1	1036-1054 bp	1.0	79.1
ycf4-cemA_p3	AAAAAGCTGCTGAATTGGCC	TAGAGAAATCCACCAGGGCA	45.0	50.0	57.8	58.0	1052-1070 bp	1.0	78.8
ycf4-cemA_p4	AAAAAGCTGCTGAATTGGCC	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	1050-1068 bp	1.0	78.4
ycf4-cemA_p5	AAAGCTGCTGAATTGGCCTA	TAGAGAAATCCACCAGGGCA	45.0	50.0	57.8	58.0	1050-1068 bp	1.0	78.4