Hotspot

accD-psaI

LSC · 60894-61562 · 669 bp

Back to Saurauia

Pi 0.00747

MarkerSeek score 54.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 669
variable_sites: 5
pi: 0.00747
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGTTCCACGCTTTCTTTCCT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.4	58.0	783-784 bp	1.0	75.2
accD-psaI_p2	CAGTTCCACGCTTTCTTTCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.3	58.0	784-785 bp	1.0	74.8
accD-psaI_p3	AGTTCCACGCTTTCTTTCCT	AGAAACCATTGCAATTGCCG	45.0	45.0	57.4	57.9	788-789 bp	1.0	74.7
accD-psaI_p4	CAGTTCCACGCTTTCTTTCC	AGAAACCATTGCAATTGCCG	50.0	45.0	57.3	57.9	789-790 bp	1.0	74.3
accD-psaI_p5	AGTTCCACGCTTTCTTTCCT	GCCGGAAATACTAGGCCTAC	45.0	55.0	57.4	57.5	772-773 bp	1.0	71.8