Hotspot

trnT (UGU)-trnL (UAA)

LSC · 47783-48636 · 854 bp

Back to Saurauia

Pi 0.00351
MarkerSeek score 60.80
Primer available yes
Label trnT (UGU)-trnL (UAA)

Feature Metadata

label_name
trnT (UGU)-trnL (UAA)
feature_type
igs
region
LSC
length_bp
854
variable_sites
3
pi
0.00351
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT (UGU)-trnL (UAA)_p1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 55.0 50.0 57.9 58.0 904-906 bp 1.0 79.2
trnT (UGU)-trnL (UAA)_p2 GACCGTCGCATTACAAATGC ACCAATTTCGCCATATCCCC 50.0 50.0 58.2 58.0 922-924 bp 1.0 78.7
trnT (UGU)-trnL (UAA)_p3 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 55.0 50.0 57.9 58.1 912-914 bp 1.0 78.6
trnT (UGU)-trnL (UAA)_p4 GACCGTCGCATTACAAATGC TAGCGTCTACCAATTTCGCC 50.0 50.0 58.2 58.1 930-932 bp 1.0 78.2
trnT (UGU)-trnL (UAA)_p5 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 55.0 50.0 57.9 58.2 918-920 bp 1.0 77.3