Hotspot

ycf1

SSC · 121712-127258 · 5547 bp

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Pi 0.0074

MarkerSeek score 42.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5547
variable_sites: 41
pi: 0.0074
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCAGGCCTCAATATCGACT	CTTCCGGGCTATGAAACGAA	50.0	50.0	57.9	58.0	517-526 bp	1.0	81.8
ycf1_p2	CCAGATTCGATATAGGGCGC	CTTCCGGGCTATGAAACGAA	55.0	50.0	58.1	58.0	700-709 bp	1.0	81.4
ycf1_p3	TGATTCAAAGTTGCGTCCCA	CTTCCGGGCTATGAAACGAA	45.0	50.0	58.0	58.0	1070-1079 bp	1.0	81.1
ycf1_p4	TGATTCAAAGTTGCGTCCCA	CCGGGCTATGAAACGAATCA	45.0	50.0	58.0	58.1	1067-1076 bp	1.0	80.9
ycf1_p5	TGATTCAAAGTTGCGTCCCA	AGTTCGCCAGCGTTGTATAG	45.0	50.0	58.0	58.1	960-969 bp	1.0	80.7