Hotspot

rps14

LSC · 36200-36502 · 303 bp

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Pi 0.0066

MarkerSeek score 56.40

Primer available yes

Label rps14

Feature Metadata

label_name: rps14
feature_type: gene
region: LSC
length_bp: 303
variable_sites: 2
pi: 0.0066
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14_p1	AAAGAACGATTGAATGCGCC	TTTTTCGATGGGGAGAAGGC	45.0	50.0	57.7	58.2	506 bp	1.0	76.9
rps14_p2	CGCCCCTCTACCATATCTGT	TTTTTCGATGGGGAGAAGGC	55.0	50.0	58.4	58.2	490 bp	1.0	76.1
rps14_p3	ACAGAATGGTAAAGAGGCCG	TTTTTCGATGGGGAGAAGGC	50.0	50.0	57.6	58.2	448 bp	1.0	75.8
rps14_p4	CAGAATGGTAAAGAGGCCGT	TTTTTCGATGGGGAGAAGGC	50.0	50.0	57.6	58.2	447 bp	1.0	75.8
rps14_p5	AATGCGCCCCTCTACCATAT	TTTTTCGATGGGGAGAAGGC	50.0	50.0	58.6	58.2	494 bp	1.0	73.9