Hotspot

ndhF

SSC · 108507-110738 · 2232 bp

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Pi 0.00941

MarkerSeek score 48.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 21
pi: 0.00941
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GGAAGAAAAGGCGGATCTGA	CTTTTAACGCCGCCCAATAG	50.0	50.0	57.6	57.5	2957-2974 bp	1.0	73.7
ndhF_p2	GGCGGATCTGAACAAAATCG	CTTTTAACGCCGCCCAATAG	50.0	50.0	57.5	57.5	2948-2965 bp	1.0	73.3
ndhF_p3	GGAAGAAAAGGCGGATCTGA	CGCCCAATAGCCTTTCCTTT	50.0	50.0	57.6	58.5	2947-2964 bp	1.0	73.2
ndhF_p4	GGAAGAAAAGGCGGATCTGA	AAAATGCTTTTAACGCCGCC	50.0	45.0	57.6	58.6	2963-2980 bp	1.0	72.8
ndhF_p5	AGGAACAAGAGGGATCCGAA	CGCCCAATAGCCTTTCCTTT	50.0	50.0	58.0	58.5	2996 bp	0.5	55.4