Hotspot

ndhD

SSC · 113514-115016 · 1503 bp

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Pi 0.00266

MarkerSeek score 48.50

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 4
pi: 0.00266
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TCCCGCCAGTTCAAATTCAA	TTTTTCTGGCCCAAGCGTAT	45.0	45.0	58.0	58.1	1662 bp	0.5	59.2
ndhD_p2	TCCCGCCAGTTCAAATTCAA	ATGTACCCAATGTGTCCGTG	45.0	50.0	58.0	57.9	1958 bp	0.5	59.0
ndhD_p3	TCCCGCCAGTTCAAATTCAA	ACTGCGTTGGTTGTAAGAGG	45.0	50.0	58.0	58.1	1857 bp	0.5	58.9
ndhD_p4	TCCCGCCAGTTCAAATTCAA	CTGCGTTGGTTGTAAGAGGT	45.0	50.0	58.0	58.1	1856 bp	0.5	58.9
ndhD_p5	TCCCGCCAGTTCAAATTCAA	GTGCCCCACCGATGTATTAG	45.0	55.0	58.0	58.1	1937 bp	0.5	58.9