Hotspot

ccsA

SSC · 112298-113275 · 978 bp

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Pi 0.00409

MarkerSeek score 47.00

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 978
variable_sites: 4
pi: 0.00409
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TAGCGGCATGGCATATCATC	TCCCTCGCATTACCAGGTAT	50.0	50.0	58.0	57.9	1675-1688 bp	1.0	79.7
ccsA_p2	TAGCGGCATGGCATATCATC	TTGAATTTGAACTGGCGGGA	50.0	45.0	58.0	58.0	1217 bp	0.5	59.7
ccsA_p3	TCTCTCGCCATCAATTCACG	TTGAATTTGAACTGGCGGGA	50.0	45.0	58.1	58.0	1687 bp	0.5	59.3
ccsA_p4	GCCGGTTTTAGAAGAGCTCA	TTGAATTTGAACTGGCGGGA	50.0	45.0	57.9	58.0	1493 bp	0.5	59.1
ccsA_p5	TAGACACGCTGCTCTTAGGA	TTGAATTTGAACTGGCGGGA	50.0	45.0	57.9	58.0	1270 bp	0.5	58.9