Hotspot

atpI

LSC · 14598-15341 · 744 bp

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Pi 0.00672

MarkerSeek score 62.60

Primer available yes

Label atpI

Feature Metadata

label_name: atpI
feature_type: gene
region: LSC
length_bp: 744
variable_sites: 5
pi: 0.00672
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	ACATATGCGTTGCTTTGCAC	TCCGATTCAAGTAGACAAAGAGA	45.0	39.1	58.0	57.0	858-859 bp	1.0	48.5
atpI_p2	CACATATGCGTTGCTTTGCA	TCCGATTCAAGTAGACAAAGAGA	45.0	39.1	58.0	57.0	859-860 bp	1.0	48.5
atpI_p3	CATATGCGTTGCTTTGCACT	TCCGATTCAAGTAGACAAAGAGA	45.0	39.1	57.7	57.0	857-858 bp	1.0	46.5
atpI_p4	ATGCGTTGCTTTGCACTAAG	TCCGATTCAAGTAGACAAAGAGA	45.0	39.1	57.7	57.0	854-855 bp	1.0	46.0
atpI_p5	TCTTGAGCCACATATGCGTT	TCCGATTCAAGTAGACAAAGAGA	45.0	39.1	57.6	57.0	867-868 bp	1.0	45.4