Hotspot

ndhD-psaC

SSC · 115017-115136 · 120 bp

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Pi 0.0588

MarkerSeek score 69.10

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 120
variable_sites: 7
pi: 0.0588
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	CCCCTGCCGTGTACAAAATA	GGTTAGAGCCCTTATGTGCC	50.0	55.0	57.9	58.0	1351-1352 bp	1.0	79.5
ndhD-psaC_p2	CGCCCAGAACTCCCATTAAA	GGTTAGAGCCCTTATGTGCC	50.0	55.0	57.9	58.0	1384-1385 bp	1.0	79.4
ndhD-psaC_p3	TATAATGCGCTTCTCCGTGG	GGTTAGAGCCCTTATGTGCC	50.0	55.0	57.9	58.0	1561-1562 bp	1.0	79.2
ndhD-psaC_p4	AAAAATAGAACCCCCTGCCG	GGTTAGAGCCCTTATGTGCC	50.0	55.0	58.2	58.0	1362-1363 bp	1.0	79.2
ndhD-psaC_p5	CCCCTGCCGTGTACAAAATA	ATGTACCCAATGTGTCCGTG	50.0	50.0	57.9	57.9	847-848 bp	1.0	79.1