Hotspot

rpl32-trnL-UAG

SSC · 111377-112117 · 741 bp

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Pi 0.0123

MarkerSeek score 66.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 741
variable_sites: 9
pi: 0.0123
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CTATTGGGCGGCGTTAAAAG	GATGATATGCCATGCCGCTA	50.0	50.0	57.5	58.0	928-933 bp	1.0	77.5
rpl32-trnL-UAG_p2	AAAGGAAAGGCTATTGGGCG	GATGATATGCCATGCCGCTA	50.0	50.0	58.5	58.0	938-943 bp	1.0	77.0
rpl32-trnL-UAG_p3	CTATTGGGCGGCGTTAAAAG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.5	57.9	875-880 bp	1.0	76.8
rpl32-trnL-UAG_p4	AAAGGAAAGGCTATTGGGCG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.5	57.9	885-890 bp	1.0	76.3
rpl32-trnL-UAG_p5	CTATTGGGCGGCGTTAAAAG	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.5	57.8	868-873 bp	1.0	76.3