Hotspot

rps19-rpl2

IRb · 83005-83064 · 60 bp

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Pi 0.0333
MarkerSeek score 68.50
Primer available yes
Label rps19-rpl2

Feature Metadata

label_name
rps19-rpl2
feature_type
igs
region
IRb
length_bp
60
variable_sites
2
pi
0.0333
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19-rpl2_p1 GGTAGATGCACGAGACCAAG CACAACCCCTTGGGGTTATC 55.0 55.0 58.1 58.2 271 bp 1.0 78.8
rps19-rpl2_p2 GGTAGATGCACGAGACCAAG GATTGTTCGTCGCCGTAGTA 55.0 50.0 58.1 57.8 202 bp 1.0 78.8
rps19-rpl2_p3 GGTAGATGCACGAGACCAAG TTTGATTGTTCGTCGCCGTA 55.0 45.0 58.1 58.2 205 bp 1.0 78.5
rps19-rpl2_p4 GGTAGATGCACGAGACCAAG TTGGGGTTATCCTGCACTTG 55.0 50.0 58.1 57.8 262 bp 1.0 78.3
rps19-rpl2_p5 GGTAGATGCACGAGACCAAG AATTTGATTGTTCGTCGCCG 55.0 45.0 58.1 57.8 207 bp 1.0 78.3