Hotspot

ycf3-trnS-GGA

LSC · 43812-44659 · 848 bp

Back to Satureja

Pi 0.00602

MarkerSeek score 62.30

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 848
variable_sites: 5
pi: 0.00602
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TGCCTCCTTTTCTCCTGAAG	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.1	58.1	1005-1020 bp	1.0	74.0
ycf3-trnS-GGA_p2	GCCTCCTTTTCTCCTGAAGT	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.1	58.1	1004-1019 bp	1.0	74.0
ycf3-trnS-GGA_p3	TGCCTCCTTTTCTCCTGAAG	AAGTCAAGGTGCGGAAAGAG	50.0	50.0	57.1	58.1	1036-1051 bp	1.0	73.8
ycf3-trnS-GGA_p4	GCCTCCTTTTCTCCTGAAGT	AAGTCAAGGTGCGGAAAGAG	50.0	50.0	57.1	58.1	1035-1050 bp	1.0	73.8
ycf3-trnS-GGA_p5	TGCCTCCTTTTCTCCTGAAG	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.1	57.8	976-991 bp	1.0	73.3