Hotspot

trnG-GCC-trnM-CAU

LSC · 35823-35980 · 158 bp

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Pi 0.0446
MarkerSeek score 78.70
Primer available yes
Label trnG-GCC-trnM-CAU

Feature Metadata

label_name
trnG-GCC-trnM-CAU
feature_type
igs
region
LSC
length_bp
158
variable_sites
7
pi
0.0446
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnM-CAU_p1 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 225-226 bp 1.0 82.1
trnG-GCC-trnM-CAU_p2 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 226-227 bp 1.0 82.1
trnG-GCC-trnM-CAU_p3 AGAAGATGCGGGTTCGATTC GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 261-262 bp 1.0 81.8
trnG-GCC-trnM-CAU_p4 GAGAAGATGCGGGTTCGATT GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 262-263 bp 1.0 81.8
trnG-GCC-trnM-CAU_p5 ATGCGGATATGGTCGAATGG GGGTAGAGCAGTTTGGTAGC 50.0 55.0 57.9 58.0 302-303 bp 1.0 81.1