Hotspot

trnH-GUG-psbA

LSC · 87-428 · 342 bp

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Pi 0.0398
MarkerSeek score 65.70
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
342
variable_sites
13
pi
0.0398
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CTGTTGCGTATTCAGCTCCT 55.0 50.0 58.1 58.0 977-992 bp 1.0 81.3
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG GAGTGGGAGCTTAGTTTCCG 55.0 55.0 58.1 58.0 1020-1035 bp 1.0 81.2
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA GAGTGGGAGCTTAGTTTCCG 50.0 55.0 57.9 58.0 1034-1049 bp 1.0 81.1
trnH-GUG-psbA_p4 AATCCACTGCCTTGATCCAC GAGTGGGAGCTTAGTTTCCG 50.0 55.0 57.9 58.0 1033-1048 bp 1.0 81.1
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC GAGTGGGAGCTTAGTTTCCG 50.0 55.0 57.9 58.0 1035-1050 bp 1.0 81.1