Hotspot

psbC

LSC · 36208-37629 · 1422 bp

Back to Sassafras

Pi 0.00141

MarkerSeek score 59.60

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 3
pi: 0.00141
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	TTCGTTTCCCAGGAAATCCG	TCATGCATTTGATCTCCTGTC	50.0	42.9	58.2	56.0	1558 bp	1.0	54.9
psbC_p2	GCAGCGGAAGATCCTGAATT	TCATGCATTTGATCTCCTGTC	50.0	42.9	58.3	56.0	1537 bp	1.0	53.8
psbC_p3	AGGTATTCGTGCTTGGATGG	TCATGCATTTGATCTCCTGTC	50.0	42.9	57.7	56.0	1481 bp	1.0	53.7
psbC_p4	TTCGTTTCCCAGGAAATCCG	CTTCATGCATTTGATCTCCTGTC	50.0	43.5	58.2	57.8	1560 bp	1.0	53.3
psbC_p5	TTCGTTTCCCAGGAAATCCG	TTCATGCATTTGATCTCCTGTC	50.0	40.9	58.2	56.8	1559 bp	1.0	52.8