Hotspot

ndhA

SSC · 124185-126399 · 2215 bp

Back to Sassafras

Pi 0.00421

MarkerSeek score 45.50

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2215
variable_sites: 14
pi: 0.00421
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CCTTGCAGCTCGTATTGTCT	TTTGGAACGAGTTGAAGGGG	50.0	50.0	58.0	58.0	2940-2942 bp	1.0	80.2
ndhA_p2	CCTTGCAGCTCGTATTGTCT	GGTTTATCGGGACCAATGCT	50.0	50.0	58.0	57.9	2885-2887 bp	1.0	80.0
ndhA_p3	AACCCAGTCACCATAGGGAA	TTTGGAACGAGTTGAAGGGG	50.0	50.0	57.9	58.0	2903-2905 bp	1.0	79.9
ndhA_p4	AACCCAGTCACCATAGGGAA	GGTTTATCGGGACCAATGCT	50.0	50.0	57.9	57.9	2848-2850 bp	1.0	79.7
ndhA_p5	CCTTGCAGCTCGTATTGTCT	GGAAAATTCGTCCACCTGGT	50.0	50.0	58.0	58.1	2482-2484 bp	1.0	79.6