Hotspot

ndhF-rpl32

SSC · 115898-117121 · 1224 bp

Back to Sassafras

Pi 0.00604

MarkerSeek score 53.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1224
variable_sites: 11
pi: 0.00604
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCGAAAGGTATGATCCATGC	GTACGTTTCTTTGGAACCGC	50.0	50.0	57.0	57.7	1288-1307 bp	1.0	71.0
ndhF-rpl32_p2	GCGAAAGGTATGATCCATGC	GGCCTGATATCCCCTTCCTT	50.0	55.0	57.0	58.5	1346-1365 bp	1.0	69.0
ndhF-rpl32_p3	GCGAAAGGTATGATCCATGC	TACGTTTCTTTGGAACCGCC	50.0	50.0	57.0	58.8	1287-1306 bp	1.0	67.3
ndhF-rpl32_p4	AGCGAAAGGTATGATCCATGC	GTACGTTTCTTTGGAACCGC	47.6	50.0	58.5	57.7	1289-1308 bp	1.0	66.8
ndhF-rpl32_p5	AGCGAAAGGTATGATCCATGC	GGCCTGATATCCCCTTCCTT	47.6	55.0	58.5	58.5	1347-1366 bp	1.0	64.8