Hotspot

clpP-psbB

LSC · 75561-75988 · 428 bp

Back to Sassafras

Pi 0.00623

MarkerSeek score 59.10

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 428
variable_sites: 4
pi: 0.00623
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CTTTTGGAACACCAATGGGC	ATATGGACAGAAAGCAGCCG	50.0	50.0	57.8	58.0	521 bp	1.0	78.9
clpP-psbB_p2	ACCCAAACTGCATCTTCCTC	ATATGGACAGAAAGCAGCCG	50.0	50.0	57.8	58.0	561 bp	1.0	78.6
clpP-psbB_p3	ATACGTCAACCCAAACTGCA	ATATGGACAGAAAGCAGCCG	45.0	50.0	57.7	58.0	569 bp	1.0	78.1
clpP-psbB_p4	TACGTCAACCCAAACTGCAT	ATATGGACAGAAAGCAGCCG	45.0	50.0	57.7	58.0	568 bp	1.0	78.1
clpP-psbB_p5	TTTGGAACACCAATGGGCAT	ATATGGACAGAAAGCAGCCG	45.0	50.0	58.3	58.0	519 bp	1.0	78.1