Hotspot

psbE-petL

LSC · 68268-69511 · 1244 bp

Back to Sassafras

Pi 0.00107

MarkerSeek score 59.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1244
variable_sites: 2
pi: 0.00107
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.9	58.5	1391-1424 bp	1.0	75.9
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.9	58.5	1389-1422 bp	1.0	75.9
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AAGCAGCCAGTAGAAAACCG	45.0	50.0	58.2	58.5	1400-1433 bp	1.0	75.0
psbE-petL_p4	ACGAATAACCAACCCGCAAT	GCAGCCAGTAGAAAACCGAA	45.0	50.0	58.2	58.5	1398-1431 bp	1.0	75.0
psbE-petL_p5	TGCTGACGAATAACCAACCC	AAGCAGCCAGTAGAAAACCG	50.0	50.0	58.2	58.5	1405-1438 bp	1.0	74.9