Hotspot

ycf3-trnS-GGA

LSC · 46706-47514 · 809 bp

Back to Sassafras

Pi 0.0033
MarkerSeek score 60.90
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
809
variable_sites
4
pi
0.0033
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TCTCCTGAGGTTGTCGGAAT TTGTGGTCGGTTGGTGAATT 50.0 45.0 58.1 57.9 1022 bp 1.0 79.3
ycf3-trnS-GGA_p2 TCTCCTGAGGTTGTCGGAAT ACGGAAAGAGAGGGATTCGA 50.0 50.0 58.1 58.2 992 bp 1.0 78.6
ycf3-trnS-GGA_p3 TCTCCTGAGGTTGTCGGAAT TTCCAATGCTACGCCTTGAA 50.0 45.0 58.1 57.8 942 bp 1.0 78.3
ycf3-trnS-GGA_p4 TCTCCTGAGGTTGTCGGAAT ATTGTGGTCGGTTGGTGAAT 50.0 45.0 58.1 57.7 1023 bp 1.0 77.5
ycf3-trnS-GGA_p5 TTCTCCTGAGGTTGTCGGAA TTGTGGTCGGTTGGTGAATT 50.0 45.0 58.3 57.9 1023 bp 1.0 77.5