Hotspot

trnE-UUC-trnT-GGU

LSC · 32959-33679 · 721 bp

Back to Sassafras

Pi 0.00462

MarkerSeek score 61.90

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 721
variable_sites: 5
pi: 0.00462
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CATGATCAACCCCCTTTCGT	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	870-872 bp	1.0	79.5
trnE-UUC-trnT-GGU_p2	CAACCCCCTTTCGTCTTACC	GAACCGATGACTTACGCCTT	55.0	50.0	58.2	58.0	864-866 bp	1.0	79.0
trnE-UUC-trnT-GGU_p3	CATGATCAACCCCCTTTCGT	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	849-851 bp	1.0	79.0
trnE-UUC-trnT-GGU_p4	CATGATCAACCCCCTTTCGT	TGGCGTTACTCTACCACTGA	50.0	50.0	57.9	58.1	846-848 bp	1.0	78.8
trnE-UUC-trnT-GGU_p5	CAACCCCCTTTCGTCTTACC	CCATGGCGTTACTCTACCAC	55.0	55.0	58.2	58.1	843-845 bp	1.0	78.6